Soybean allergy: IgE epitopes of glycinin (Gly m 6), an important soybean allergen
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منابع مشابه
Soybean allergy: IgE epitopes of glycinin (Gly m 6), an important soybean allergen
Methods 11 subjects with clinically confirmed soybean allergy and sIgE against rGly m 6 were enrolled in this study. Synthetic overlapping peptides (15-mers, 4 aa offset) representing the amino acid sequences of G1 and G5, two representative subunits of Gly m 6, were spotted onto CelluSpotTM slides and analyzed for IgE-binding, respectively. The sequences of IgE-binding peptides were allocated ...
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Methods Sera from 33 birch pollen-allergic patients with and without clinical reactivity to soy as determined by oral food challenge or convincing history of soy allergy were included in the study. Specific IgE against Bet v 1 and Gly m 4 was determined by ImmunoCAP. IgE binding of the sera to rGly m 4 and soy extract was tested in western blot analysis. To predict putative IgE epitopes, 20 IgE...
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A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose...
متن کاملTargeting a Cross-Reactive Gly m 5 Soy Peptide as Responsible for Hypersensitivity Reactions in a Milk Allergy Mouse Model
BACKGROUND Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major soybean allergen Gly m 5 that are co-recognized by casein specific antibodies, and to identify a peptide responsible for the cross-reactivity. METHODS Cow's milk protein (CMP)-specific antibodies were used in different immunoassays (immunob...
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The soybean allergenic protein, Gly m Bd 30K [Ogawa et al., J. Nutr. Sci. Vitaminol., 37, 555-565 (1991)] which is most strongly and frequently recognized by the IgE antibodies in sera of soybean-sensitive patients with atopic dermatitis, has been characterized. The allergen was isolated from the crude 7S-globulin fraction as an oligomeric form with a molecular weight of more than 3000,000 by g...
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ژورنال
عنوان ژورنال: Clinical and Translational Allergy
سال: 2014
ISSN: 2045-7022
DOI: 10.1186/2045-7022-4-s2-o2